METHOD FOR PRODUCING RECOMBINANT THROMBIN

Preparing folded prothrombin or its derivatives, comprises solubilizing inclusion bodies containing unfolded prothrombin or its derivatives in a solubilization buffer comprising at least a chaotropic compound and at least an organic disulfide compound. Independent claims are included for: (1) a proc...

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Bibliographische Detailangaben
Hauptverfasser:	KOETTER, JOCHEN, SCHAEFFNER, JOERG, ANTON, ANDREAS, DIETRICH, ARNDT
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Preparing folded prothrombin or its derivatives, comprises solubilizing inclusion bodies containing unfolded prothrombin or its derivatives in a solubilization buffer comprising at least a chaotropic compound and at least an organic disulfide compound. Independent claims are included for: (1) a process for producing thrombin and/or alpha -thrombin or its derivatives, comprising converting a renatured prothrombin obtained by the above process by enzymatic proteolysis with ecarin to thrombin and/or alpha -thrombin or its derivatives; and (2) a solution containing prothrombin, folded thrombin and/or folded alpha -thrombin or their derivatives, obtained by the above process.