BRANCHED-CHAIN FATTY ACIDS AND BIOLOGICAL PRODUCTION THEREOF

BRANCHED-CHAIN FATTY ACIDS AND BIOLOGICAL PRODUCTION THEREOF

A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: GREEN, PHILLIP, RICHARD, XU, JUN, CODY, DAVID, BLAIR, KHAMBATTA, ZUBIN, SAROSH, SAUNDERS, CHARLES, WINSTON
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to -methyl-D-malate; conversion of -methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to α-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided.