Method of obtaining inhibitors of cysteine peptidases with an electrophoretic purity from biological materials

A method of producing cysteine peptidase inhibitors of electrophoretic purity from biological materials characterised in that it encompasses: a) the preparation of a protein mass to yield cysteine peptidase inhibitors by acting on a protein mass solution with a n acidic or basic pH, preferably close to pH 2, as well as a high temperature, preferably 90°C for 10 to 20 minutes, after which the protein mass solution is preferably frozen for several hours, preferably 12; b) the production of cysteine peptidase inhibitors through at least one affinity chromatography, preferably on a column, against carriers with bound cysteine peptidase, preferably papain or phycin or another derivative of plant cysteine peptidases using the preferred, defrosted protein mass of stage a) with an activity of at least 5 IU/mg in the eluent following the first elution, wherein the reclamation of inhibitors bound to the peptide is conducted with a solution, preferably with 20% glycerol, with a pH of from 9.5 to 12 at a temperature from 8°C to 55°C during multiple usage of the chromatography gel, or at a temperature of from 60°C to 100°C with single usage of the chromatography gel.