METHOD FOR DETECTION OF TARGET NUCLEIC ACID

An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer 30 comprising a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	NIWA, Kousuke, HIROTA, Toshikazu, KAWASE, Mitsuo
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer 30 comprising an identification sequence 32 complementary to a target sequence 12 in a target nucleic acid and a tag addition sequence 36, and a second primer 40 having a label 42 are prepared. The first primer 30 and the second primer 40 are used for the target nucleic acid 10 in a sample to amplify a chimeric DNA 60 having a tag sequence 66 and the label 42. The chimeric DNA 60 is hybridized with a detection probe 104 on a solid phase 100 to obtain signal intensity information based on the label 42, and the target nucleic acid 10 is detected based on the signal intensity information.