COMPENSATION FOR SPECTRAL CROSSTALK IN MULTIPLEX NUCLEIC ACID AMPLIFICATION

Compensation for spectral crosstalk in multiplex nucleic acid amplification is disclosed, comprising: a calibration process comprising performing at least a single-plex nucleic acid amplification of a nucleic acid sample using a detection probe, wherein the nucleic acid amplification occurs over a plurality of interrogation periods; from the nucleic acid amplification, acquiring amplification data that indicates an amount of nucleic acid present for each of the plurality of interrogation periods; and based on the amplification data, determining a crosstalk correction value associated with a spectral neighbor to the detection probe to reduce spectral crosstalk from the spectral neighbor; and a nucleic acid amplification analysis including applying the crosstalk correction value obtained from the calibration process to amplification data collected from multiplex nucleic acid amplification of nucleic acid samples.