Optical detection system for a microfluidic device and method for aligning and focusing an optical detection system

An optical detection system (100) for a microfluidic device (165) is described. The system (100) includes a microfluidic device (165) including alignment marks; an LED (110); means for collimating light emitted by the LED (110); an objective lens (160); means for directing the collimated light throu...

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Bibliographische Detailangaben
Hauptverfasser:	Wolk, Jeffrey A, Slater, Michael, Jensen, Morten J, Lee, Ernest C. W, Rulison, Aaron
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING ANGLES MEASURING AREAS MEASURING IRREGULARITIES OF SURFACES OR CONTOURS MEASURING LENGTH, THICKNESS OR SIMILAR LINEARDIMENSIONS OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	An optical detection system (100) for a microfluidic device (165) is described. The system (100) includes a microfluidic device (165) including alignment marks; an LED (110); means for collimating light emitted by the LED (110); an objective lens (160); means for directing the collimated light through the objective onto the microfluidic device (165); and means (150) for detecting a signal emitted from the microfluidic device (165). The working distance between the objective (160) and the device (165) allows light from an external LED (110) or laser to be brought in along a diagonal path to illuminate the microfluidic device (165). The microfluidic device (165) includes multiple channels and multiple closed optical alignment marks having curved walls. The marks are illuminated for alignment and focusing purposes by light brought in on a diagonal path from an external LED (110).