CAPTURE AND ELUTION OF BIO-ANALYTES VIA BEADS THAT ARE USED TO DISRUPT SPECIMENS

Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a...

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Bibliographische Detailangaben
Hauptverfasser: TALBOT, RYAN, P, ERWIN, BARBARA, NIEMZ, ANGELIKA, BRIGITTE, DOEBLER, ROBERT, W, IRVINE, BRUCE
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Heating may be used. Lysing may be performed in a flow through apparatus.