SPIM microscope with STED light sheet
The microscope has an illumination source (4) sending an illuminating light beam from a y-direction on an object (2). An x-scanner (12) sequentially produces a light sheet (1) by scanning of the light beam in an x-direction. The x-direction is extended vertically to a y-direction and to the z-direct...
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| Format: | Patent |
| Sprache: | eng ; fre ; ger |
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| Zusammenfassung: | The microscope has an illumination source (4) sending an illuminating light beam from a y-direction on an object (2). An x-scanner (12) sequentially produces a light sheet (1) by scanning of the light beam in an x-direction. The x-direction is extended vertically to a y-direction and to the z-direction. The light sheet is constructed according to a sequential plane fixed from the x -direction and the y-direction. An illumination optics is provided with a zoom optics (13) arranged in an optical path of the light beam for varying a focus length of the light beam. A camera serves as a complementary MOS camera and a charge coupled device to detect fluorescence light and/or reflection light. |
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