Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene

Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a V²-J²...

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Hauptverfasser: MACINTYRE, ELIZABETH ANNE, KNEBA, MICHAEL, BASTARD, CHRISTIAN, EVANS, PAUL ANTHONY STUART, BRUEGGEMANN, MONIKA, SMITH, JOHN LEWIS, LANGERAK, ANTHONIE WILLEM, SCHUURING, EDUARDUS MARIA DOMINICUS, MORGAN, GARETH JOHN, GARCIA SANZ, RAMON, HUMMEL, MICHAEL, VAN DONGEN, JACOBUS JOHANNES MARIA, PARREIRA, ANTONIO, LAVENDER, FRANCES LOUISE, DAVI, FREDERIC BERNARD LOUIS, SAN MIQUEL, JESUS FERNANDO
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a V²-J² TCRB rearrangement, and optionally a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V»-J» IGL rearrangement, a D²-J² TCRB rearrangement, a V³-J³ TCRG rearrangement, a V´-J´ TCRD rearrangement, a D´-D´ TCRD rearrangement, a D´-J´ TCRD rearrangement, a V´-D´ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-ICrH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.