METHOD FOR RENDERING THE MEMBRANES OF BIOLOGICAL CELLS PERVIOUS BY USING A PULSED ELECTRIC FIELD

A method for membrane permeabilization of biological cells contained in a product is disclosed. According to one aspect, the method is applied in a treatment device including at least one treatment chamber emitting a pulsed electric field. According to another aspect, the method includes supplying t...

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Bibliographische Detailangaben
Hauptverfasser: SCHRIVE, LUC, PUJOL, FLORIAN, LUMIA, GUY
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A method for membrane permeabilization of biological cells contained in a product is disclosed. According to one aspect, the method is applied in a treatment device including at least one treatment chamber emitting a pulsed electric field. According to another aspect, the method includes supplying the treatment device with a product including biological cells at a predetermined supply flow rate from a supply unit, introducing the product including the biological cells into the treatment chamber at an introduction flow rate, treating the product introduced into the chamber with a pulsed electric field, and reintroducing the treated product, at a predetermined feedback flow rate at a point that is downstream from the supply unit. According to some aspects, the introduction flow rate includes the feedback flow rate and the supply flow rate.