Invention related to flocculation of yeast cells

Flocculation protein Flo5 having N-terminal A-domain according to sequence ID NO: 1 (comprising a fully defined 249 amino acid sequence, as given in the specification), is new. Independent claims are included for: (1) a yeast cell comprising the flocculation protein Flo5; and (2) a method for detect...

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Bibliographische Detailangaben
Hauptverfasser:	BRUECKNER, STEFAN, MOESCH, HANS-ULRICH, VEELDERS, MAIK, ESSEN, LARS-OLIVER
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Flocculation protein Flo5 having N-terminal A-domain according to sequence ID NO: 1 (comprising a fully defined 249 amino acid sequence, as given in the specification), is new. Independent claims are included for: (1) a yeast cell comprising the flocculation protein Flo5; and (2) a method for detecting Flo5A-carrying cells, comprising immobilizing the yeast cells on a microtiter plate and detecting Flo5A-domain specific antibody by a marked secondary antibody that recognizes and binds to specific epitopes of the Flo5A domain-specific antibody. Die vorliegende Erfindung beschreibt Mutationen in der Flo5A-Domän Flokkulinen industrieller Hefen, die zu einer gezielten Modifikation der Substratspezifität und von Flokkulinproteinen führen. Insbesondere Flo5A-Q98A Mutante zeigt überraschenderweise in vivo einen NewFlo -Typ der Flokkulationsinhibition, durch eine Inhibition auch durch Glucose, ohne jedoch insgesamt an Flokkulationsfähigkeit zu verlieren. Weiterhin wird eine Verfahren zur Detektion Flo5-tragender Hefezellen in einer Zellsuspension bereitgestellt.