Method for isolating small RNA-molecules using HC-Pro protein

The present invention relates to a method for isolating small RNA-molecules (sRNAs) from a sample using Helper-component-protein (HC-Pro) from potyviruses. The present invention furthermore relates to an improved method for isolating sRNAs from a sample using HC-Pro together with a cupin protein or...

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Bibliographische Detailangaben
Hauptverfasser:	KRCZAL, GABRIELE, DR, FUELLGRABE, MARC, KAJOHN, BOONROD, WASSENEGGER, MICHAEL
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for isolating small RNA-molecules (sRNAs) from a sample using Helper-component-protein (HC-Pro) from potyviruses. The present invention furthermore relates to an improved method for isolating sRNAs from a sample using HC-Pro together with a cupin protein or the cupin 2-superfamily domain thereof. The methods of the present invention can be used in the treatment, diagnosis and monitoring of diseases, for screening compounds modifying the number, amount, and/or concentration of sRNAs. Finally, the invention also relates to a kit for isolating sRNAs from a sample.