Methods and systems for detecting multiple fluorescent emission signals

A method for detecting two or more different optical emission signals emitted from each of a plurality of reaction receptacles, said method comprising, for each optical emission signal to be detected, the steps of: (a) directing an optical excitation signal at each reaction receptacle, the optical e...

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Bibliographische Detailangaben
Hauptverfasser: Haito, Li, Scalese, Robert F, Hayes, Matthew J, Knight, Byron J
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A method for detecting two or more different optical emission signals emitted from each of a plurality of reaction receptacles, said method comprising, for each optical emission signal to be detected, the steps of: (a) directing an optical excitation signal at each reaction receptacle, the optical excitation signal having a known wavelength that excites an associated optical emission signal having a known emission wavelength, wherein the optical excitation signal has a known excitation frequency; (b) detecting an optical signal which includes any optical emission signal emitted by the contents of each receptacle; and (c) discarding any portion of the detected optical signal having a frequency that is inconsistent with the known excitation frequency to determine the portion of the detected optical signal that corresponds to the associated optical emission signal.