Enzymatic encoding methods for efficient synthesis of large libraries

Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reactio...

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Bibliographische Detailangaben
Hauptverfasser:	HOLTMANN, ANETTE, KALDOR, DITTE KIVSMOSE, PETERSEN, LENE, HANSEN, ANDERS HOLM, SOERENSEN, ANDERS MALLING, DOLBERG, JOHANNES, DE LEON, DAEN, FRANCH, THOMAS, JAKOBSEN, SOEREN NYBOE, NOERREGAARD-MADSEN, MADS, GODSKESEN, MICHAEL ANDERS, SLOEK, FRANK ABILDGAARD, JENSEN, KIM BIRKEBAEK, ANDERSEN, ANNE LEE, HUSEMOEN, BIRGITTE NYSTRUP, OLSEN, EVA KAMPMANN, GOLDBECH, ANNE, NEVE, SOEREN, LUNDORF, MIKKEL DYBRO, GLAD, SANNE SCHROEDER
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).