USE OF MONOOLEIN AS A NEW AUXILIARY LIPID IN LIPOFECTION

The present invention relates to the application of monoolein-based cationic lipid systems for the complexation, transport and transfection of genetic material into target cells. The lipid systems are prepared through the conjugation of the neutral lipid monooleoyl-rac glycerol (Monoolein) with the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	CUNHA DIAS REAL OLIVEIRA, Maria Elisabete, P. N. SILVA, João, FERREIRA CASTRO GOMES, Andreia, FERNANDES PEREIRA COUTINHO, Olga Maria, GOMES COUTINHO, Paulo José, PEDRA AMORIM CASAL, Margarida Paula
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention relates to the application of monoolein-based cationic lipid systems for the complexation, transport and transfection of genetic material into target cells. The lipid systems are prepared through the conjugation of the neutral lipid monooleoyl-rac glycerol (Monoolein) with the cationic tensioactives derived from Dioctadecyldimethylammonium (DODAX) as, for example, Dioctadecyldimethylammonium Bromide (DODAB), Dioctadecyldimethylammonium Chloride (DODAC) and Dioctadecyldimethylammonium Phosphate (DODAF). The lipofection method here describe, presenting high levels of biocompatibility and reduced acquisition costs, is applicable for molecular biology purposes ( in vitro application), as well as in the health area in gene therapy ( i n vivo application).