METHOD FOR PRODUCING FIBROPLAST CELLS FROM THE NEWBORN'S NAVEL-CORD

METHOD FOR PRODUCING FIBROPLAST CELLS FROM THE NEWBORN'S NAVEL-CORD

The invention relates to bioengineering. The inventive method for producing fibroblast-like cells from the newborn's umbilical cord involves preparing the fragment of the newborn's umbilical cord, homogenising, extracting the fibroplast cells by treating with a solution containing a type-I...

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Bibliographische Detailangaben
Hauptverfasser: KISELJOV, Sergejj L'vovich, LAGAR'KOVA, Marija Andreevna, PRIKHOD'KO, Aleksandr Viktorovich, ISAEV, Artur Aleksandrovich, MELIKHOVA, Varvara Sergeevna, KOSHELEVA, Nastas'ja Vladimirovna, SABURINA, Irina Nikolaevna
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to bioengineering. The inventive method for producing fibroblast-like cells from the newborn's umbilical cord involves preparing the fragment of the newborn's umbilical cord, homogenising, extracting the fibroplast cells by treating with a solution containing a type-I collagenase and a type IV collagenase with an enzyme ratio of 1:1 in the proportion of homogenate to the enzyme solution of 1:5. The method makes it possible to increase the yield of fibroblast cells, is easily used and allows to cryo-preserve the cells in 25-30 days after receiving material at a laboratory.