Method for removing paraffin from biological samples

Isolating biomolecules from fixed biological sample, which is embedded in paraffin, comprises (a) contacting the sample with a water-immiscible solvent and dissolving the paraffin in the solvent to form a liquid organic phase, (b) contacting the sample with an aqueous solution of at least one lytic...

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Bibliographische Detailangaben
Hauptverfasser:	Meusel, Markus, Radmacher, Edmund, Beyard, Claudia, Kirsch, Christoph
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Isolating biomolecules from fixed biological sample, which is embedded in paraffin, comprises (a) contacting the sample with a water-immiscible solvent and dissolving the paraffin in the solvent to form a liquid organic phase, (b) contacting the sample with an aqueous solution of at least one lytic substance and/or with water and the lytic substance to form an aqueous phase, where a two-phase mixture is formed by the steps (a) and (b), and (c) isolating the biomolecules from the aqueous phase. An independent claim is included for a kit, for the isolation of biomolecules from a fixed biological sample that is embedded in paraffin, comprising the water-immiscible solvent, and the lytic substance and/or the aqueous solution of lytic substance.