MCSP-aligned scFv antibody fragments and use of same

Single-chain fragment variable (scFv) antibody fragment (I), directed against melanoma-associated chondroitin sulfate proteoglycan (MCSP), with variable domains in an orientation of heavy chain variable domain-linker-light chain variable domain (VH-linker-VL), is new, where the VH domain comprises a...

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Hauptverfasser: SCHULER, GEROLD DR, SCHWENKERT, MICHAEL, SCHAFT, NIELS, DOERRIE, JAN
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Single-chain fragment variable (scFv) antibody fragment (I), directed against melanoma-associated chondroitin sulfate proteoglycan (MCSP), with variable domains in an orientation of heavy chain variable domain-linker-light chain variable domain (VH-linker-VL), is new, where the VH domain comprises amino acid sequences of SEQ ID NO. 1 (comprising a fully defined 121 amino acid sequence, as given in the specification) or SEQ ID NO. 2 (comprising a fully defined 121 amino acid sequence, as given in the specification) and the linker is a covalent bond or an oligopeptide. Single-chain fragment variable (scFv) antibody fragment (I), directed against melanoma-associated chondroitin sulfate proteoglycan (MCSP), with variable domains in an orientation of heavy chain variable domain-linker-light chain variable domain (VH-linker-VL), is new, where the VH domain comprises amino acid sequences of SEQ ID NO. 1 (comprising a fully defined 121 amino acid sequence, as given in the specification) or SEQ ID NO. 2 (comprising a fully defined 121 amino acid sequence, as given in the specification), the VL domain comprises amino acid sequences of SEQ ID NO. 3 (comprising a fully defined 112 amino acid sequence, as given in the specification) or SEQ ID NO. 4 (comprising a fully defined 112 amino acid sequence, as given in the specification) or corresponding sequences exhibiting one or more point mutations, and the linker is a covalent bond or an oligopeptide. Independent claims are included for: (1) a fusion protein comprising (i) a domain with (I) and (ii) at least a domain with a functional protein- or peptide sequence that is covalently linked with the domain (i); (2) nucleic acid sequences for (I) or the fusion protein; (3) a vector comprising the nucleic acid sequences; and (4) a cell comprising (I), fusion protein, the vector and/or the nucleic acid sequences. ACTIVITY : Cytostatic. MECHANISM OF ACTION : None given. Die Erfindung betrifft gegen das Melanom-assoziierte Chondroitinsulfat Proteoglykan (MCSP)-gerichtete scFv-Antikörperfragmente mit spezifischer Anordnung der variablen Domänen und spezielle Fusionsproteine dieser scFv-Fragmente, deren Herstellung und Verwendung zur behandlung von Tumorerkrankungen.