METHOD AND USE OF DEVICE FOR PRODUCING BIOMOLECULES

A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation...

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Bibliographische Detailangaben
Hauptverfasser:	URTHALER, Jochen, ASCHER, Christine, BUCHELI, Daniel
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DIFFERENTIAL SEDIMENTATION ENZYMOLOGY FLOTATION MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO 2 bubbles on the precipitate floc. The CO 2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of flocs and clarified lysate).