Novel polymer and method for determining cholesterol using the same

It is an object of the present invention to provide the method for determining cholesterol in lipoprotein and reagent for determining cholesterol comprising the polymer, and the present invention relates to the reagent for determining cholesterol in lipoproteins such as high-density lipoprotein (HDL...

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Bibliographische Detailangaben
Hauptverfasser:	Koyama, Isao, Yagura, Yo, Yamamoto, Naoyuki, Itai, Tomokazu, Mori, Kiyoko
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	It is an object of the present invention to provide the method for determining cholesterol in lipoprotein and reagent for determining cholesterol comprising the polymer, and the present invention relates to the reagent for determining cholesterol in lipoproteins such as high-density lipoprotein (HDL), low-density lipoprotein(LDL) and very low-density lipoprotein(VLDL) and the determination method using the polymer comprising the following units as constituents: (iv) a polyethylene glycol segment represented by the general formula [1]: €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ(CH 2 CH 2 O)k-€ƒ€ƒ€ƒ€ƒ€ƒ[1] (wherein, k represents an integer of 10 to 250); (v) a monomer unit represented by the general formula [2]: (wherein R 1 represents a hydrogen atom or C 1 -C 3 alkyl group); and (vi) a monomer unit represented by the general formula [3]: [wherein, R 2 represents a hydrogen atom or C 1 -C 3 alkyl group, and R 3 represents a group represented by the general formula [4]: €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ-COOR 4 €ƒ€ƒ€ƒ€ƒ€ƒ[4] (wherein, R 4 represents an alkyl group, a haloalkyl group or a bornyl group), an alkyl group, an alkoxy group, an aralkyl group or an alkylcarbamoyl group; particularly, in the direct measurement of cholesterols such as HDL, LDL and VLDL contained in the sample, the reagent for determining cholesterol in the presence of said polymer, and the method for determining the concentration of cholesterol in a specific lipoprotein using said reagent for determining cholesterol, and a novel polymer.