Deubiquination assays

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing deubiquination, activity using resonance energy transfer (RET) are provided. Either a substrate is linked to donor and acceptor (at least one of which is a fluorescent polypeptide) or a substrate i...

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Hauptverfasser: HUWILER, KRISTIN G, VOGEL, KURT WILLIAM, ROBERS, MATTHEW BRIAN, VEDVIK, KEVIN, MICHAUD, GREGORY ALLEN, MACHLEIDT, THOMAS, HORTON, ROBERT ARON, RIDDLE, STEVEN MICHAEL
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creator HUWILER, KRISTIN G
VOGEL, KURT WILLIAM
ROBERS, MATTHEW BRIAN
VEDVIK, KEVIN
MICHAUD, GREGORY ALLEN
MACHLEIDT, THOMAS
HORTON, ROBERT ARON
RIDDLE, STEVEN MICHAEL
description Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing deubiquination, activity using resonance energy transfer (RET) are provided. Either a substrate is linked to donor and acceptor (at least one of which is a fluorescent polypeptide) or a substrate is linked to either donor or acceptor and the other FRET partner is present on a binding partner capable of recognising the ubiquitination state of a substrate. Corresponding fusion polypeptide constructs are claimed as products.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title Deubiquination assays
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