Deubiquination assays

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing deubiquination, activity using resonance energy transfer (RET) are provided. Either a substrate is linked to donor and acceptor (at least one of which is a fluorescent polypeptide) or a substrate i...

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Bibliographische Detailangaben
Hauptverfasser:	HUWILER, KRISTIN G, VOGEL, KURT WILLIAM, ROBERS, MATTHEW BRIAN, VEDVIK, KEVIN, MICHAUD, GREGORY ALLEN, MACHLEIDT, THOMAS, HORTON, ROBERT ARON, RIDDLE, STEVEN MICHAEL
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing deubiquination, activity using resonance energy transfer (RET) are provided. Either a substrate is linked to donor and acceptor (at least one of which is a fluorescent polypeptide) or a substrate is linked to either donor or acceptor and the other FRET partner is present on a binding partner capable of recognising the ubiquitination state of a substrate. Corresponding fusion polypeptide constructs are claimed as products.