ASSEMBLY OF LARGE NUCLEIC ACIDS

A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.

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Bibliographische Detailangaben
Hauptverfasser: VENTER, J., Craig, YOUNG, Lei, BENDERS, Gwynedd, A, GIBSON, Daniel, G, SMITH, Hamilton, O, HUTCHISON, Clyde, A, GLASS, John, I
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.