Antibodies for determining the prothrombin fragment F2/F1+2 in a homogenous immunoassay

Isolated immune complex containing a peptide that contains at least a complete prothrombin fragment F2, where an antibody fragment with a specificity for the carboxy terminal neoepitope of the prothrombin fragment F2/F1 + 2 is bonded on its carboxy terminal, is new. Independent claims are included for: (1) a monoclonal antibody, which binds to an immune complex, but not to the prothrombin fragment F2 alone, and not to the antibody fragment with a specificity for the carboxy terminal neoepitope of the prothrombin fragment F2/F1 + 2 alone; (2) a hybridoma cell line that produces a monoclonal antibody; (3) a reagent containing a monoclonal antibody or its fragment; (4) a test kit containing a reagent; and (5) determining the prothrombin fragment F1 + 2 in a sample, comprising contacting the sample with an antibody or its fragment with a specificity for the carboxy terminal neoepitope of the prothrombin fragment F2/F1 + 2 for the formation of the immune complex with the prothrombin fragment F1 + 2 contained in the sample, where the sample is also contacted with the monoclonal antibody. Die vorliegende Erfindung liegt auf dem Gebiet der Gerinnungsdiagnostik und betrifft Antiköper, die spezifisch an einen Immunkomplex aus Prothrombin-Fragment F2/F1+2 und einem F2/F1+2-Neoepitop-spezifischen Antikörperfragment binden, sowie deren Herstellung und Verwendung in Verfahren zur Bestimmung von F2/F1+2.