Method for obtaining short RNA and kit for same

Extracting at least a short RNA comprises: providing a biological solution containing short RNA and proteins and/or long nucleic acids, preferably RNA and DNA; separating the proteins and long nucleic acids from the solution, where the proteins are precipitated; adsorbing the short RNA at a solid (f...

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Bibliographische Detailangaben
Hauptverfasser:	RADMACHER, EDMUND, MEUSEL, MARKUS, KRIEGER, ROBERT, MOELLER, KLAUS
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Extracting at least a short RNA comprises: providing a biological solution containing short RNA and proteins and/or long nucleic acids, preferably RNA and DNA; separating the proteins and long nucleic acids from the solution, where the proteins are precipitated; adsorbing the short RNA at a solid (first) carrier after precipitating the proteins; and extracting the short RNA by desorption from the carrier. An independent claim is included for a kit for extracting the short RNA comprising at least a solid carrier for adsorption of nucleic acids, protein precipitating reagent, at least one binding substance for selective adsorption of short RNA at a solid carrier, and a manual with procedural steps.