METHODS OF RELEASING SPOROCYSTS FROM OOCYSTS USING CONTROLLED SHEAR FORCES

Methods of releasing sporocysts/sporozoites from oocysts are provided wherein a solution of oocysts is subjected to controlled shear forces sufficient to rupture the oocysts walls and release sporocysts/sporozoites therefrom. A solution of oocysts is passed through a Microfluidizer(R) processor cham...

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Bibliographische Detailangaben
Hauptverfasser:	WILSON, KERRIANNE, HARRIS, KELLY MICHELLE, HUTCHINS, JAMES EARL, HARTMAN, ANGELA
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	Methods of releasing sporocysts/sporozoites from oocysts are provided wherein a solution of oocysts is subjected to controlled shear forces sufficient to rupture the oocysts walls and release sporocysts/sporozoites therefrom. A solution of oocysts is passed through a Microfluidizer(R) processor chamber unit under defined conditions of chamber diameter, chamber geometry, and pressure. Oocysts impact the wall of the chamber and are subjected to controlled, high shear forces, tearing open the oocyst wall and releasing the sporocysts/sporozoites intact.