SUBSTANCE FOR RESTORING NORMAL CO-EXPRESSION AND INTERACTION BETWEEN LOX AND NRAGE PROTEINS

Use of a substance (I) modulating the expression and/or the activity of LOX having a sequence (SEQ ID No. 1: Not defined) and/or modulating the expression and/or the activity of NRAGE having a sequence (SEQ ID No. 2: Not defined) for making a composition to modulate the interaction between LOX and NRAGE proteins and to regulate the equilibrium between the phenomena of cell proliferation, differentiation and apoptosis, in particular in the cases where the interaction between LOX and NRAGE is absent or altered. Independent claims are included for: (1) an identification of an active ingredient that modulates the interaction between LOX and NRAGE, to improve the interaction between LOX and NRAGE when the interaction is absent or altered and to prevent or treat a state in which cellular equilibrium between proliferation, differentiation and apoptosis, is absent or altered, comprising contact of the active ingredient with living cells that are able to express the protein LOX having a sequence comprising (SEQ ID No. 1: Not defined) and/or protein NRAGE having a sequence comprising (SEQ ID No. 2: Not defined) and expression analysis of LOX and/or NRAGE, to identify an active ingredient modulating the expression and/or the activity of LOX and/or NRAGE to improve cellular equilibrium between proliferation, differentiation and apoptosis; (2) preparation of a composition comprising identification of the active ingredient using the identification method and mixing the active ingredient with an excipient to form a cosmetic, neutraceutic, dermopharmaceutic or pharmaceutical composition to prevent or treat a state in which cellular equilibrium between proliferation, differentiation and apoptosis is absent or altered; and (3) localization method of NRAGE, comprising use of anti-NRAGE antibody to detect and locate the presence of NRAGE, in a rebuilt skin model comprising the keratinocytes, or a skin section, preferably a skin section from a person whose epidermis presents a state in which cellular equilibrium between the proliferation, differentiation and apoptosis, is absent or altered. ACTIVITY : Tranquilizer; Dermatological; Antiinflammatory; Antipruritic; Immunosuppressive; Antipsoriatic; Cytostatic. MECHANISM OF ACTION : LOX gene expression modulator; NRAGE gene expression modulator. The ability of (I) to express messenger RNA of LOX by keratinocytes was tested. The results showed that (I) significantly expressed the messenger RNA coding LOX.