PREPARATION OF TEMPLATES FOR NUCLEIC ACID SEQUENCING

The present invention relates to a method of generating a template for a nucleic acid sequencing reaction comprising, (i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end, (ii) cleaving one or both strands of the double-stranded nucleic acid...

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Bibliographische Detailangaben
Hauptverfasser:	RIGATTI, ROBERTO, RASOLONJATOVO, ISABELLE MARIE J, MILTON, JOHN, SMITH, GEOFFREY PAUL, EARNSHAW, DAVID JAMES, OST, TOBIAS WILLIAM BARR, LIU, XIAOHAI, TURCATTI, GERARDO, WORSLEY, GRAHAM JOHN, WU, XIAOLIN, BARNES, COLIN, ROMIEU, ANTHONY
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method of generating a template for a nucleic acid sequencing reaction comprising, (i) providing at least one double-stranded nucleic acid molecule attached to a solid support at the 5' end, (ii) cleaving one or both strands of the double-stranded nucleic acid molecule at an abasic site, and (iii) subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.