GLUTAMATE DECARBOXYLASE MUTANT

Thermally stable GAD65 mutants are provided, which are useful for early diagnosis of insulin-dependent diabetes mellitus and retain antigenicity to pancreatic islet-²-cell-specific autoantibodies. Specifically, error-prone PCR is performed using a GAD65 gene as a template. A host microorganism is tr...

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Hauptverfasser:	YOSHIDA, MICHITERU, TANNO, KAZUNOBU, FUKASAWA, KENJI, UEDA, TETSUYA
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Thermally stable GAD65 mutants are provided, which are useful for early diagnosis of insulin-dependent diabetes mellitus and retain antigenicity to pancreatic islet-²-cell-specific autoantibodies. Specifically, error-prone PCR is performed using a GAD65 gene as a template. A host microorganism is transformed with the thus obtained random gene library. Screening is performed using as an indicator the presence or the absence of antigenicity of the expressed protein to a pancreatic islet-²-cell-specific autoantibody after heat treatment. Thus, a GAD65 mutant having thermostability and antigenicity can be obtained.