THE OPTIMUM METHOD OF AMPLIFICATION ON POLYMERASE CHAIN REACTION

The present invention discloses a method for optimizing PCR amplification by adding element material into PCR system, wherein the element material is selected from a group consisting of element gold, element titanium, element nickel, element bismuth, element stibium, element selenium, element chromi...

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Bibliographische Detailangaben
Hauptverfasser: FAN, CHUNBAI, ZHANG, ZHIZHOU, LV, JUNHONG, HUANG, JIEHUAN, LI, HAIKUO, ZHANG, XIAODONG, HU, JUN
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention discloses a method for optimizing PCR amplification by adding element material into PCR system, wherein the element material is selected from a group consisting of element gold, element titanium, element nickel, element bismuth, element stibium, element selenium, element chromium, and mixtures thereof. The method comprises: (1) preparation of the element material for optimizing; (2) sterilization of the element material; (3) the optimization of PCR system; (4) analysis of PCR products; (5) material for optimizing is removed from reaction system.