GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID

GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID

The invention provides flagellin-based fusion proteins (FBFP) are useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LOUW, MAUREEN, ELIZABETH, CRAMPTON, MICHAEL, CRAIG, BERGER, ELDIE
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Beschreibung
Zusammenfassung:The invention provides flagellin-based fusion proteins (FBFP) are useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacterial are useful for expressing the recombinant FBFP on their surfaces. Also included in the invention genetic constructs are useful for making FBFP and methods of using the FBFP.