A NOVEL PROCESS FOR PURIFICATION OF HUMAN GROWTH HARMONE

Adding enough organic solvent to hydrophobic interactive chromatography elution buffer eliminates the need to also add detergent to the buffer when separating poly-peptides. For example, adding about 50% acetonitrile to detergent-free elution buffer enaibls one to separate full-length human growth h...

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Bibliographische Detailangaben
Hauptverfasser:	BORBHUIYA, AHMED MONSUR, RAO, LAXMI SRINIVAS, MISHRA, SHRIKANT, NIPHADKAR, PRABHAKAR MILIND, MAITI, DIPANWITA, RAO, YADA MADHAVA
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	Adding enough organic solvent to hydrophobic interactive chromatography elution buffer eliminates the need to also add detergent to the buffer when separating poly-peptides. For example, adding about 50% acetonitrile to detergent-free elution buffer enaibls one to separate full-length human growth hormone from its various truncated forms, to obtain hGH with a purity of >99.5%. This technique is useful to purify polypeptide where detergent to contamination in the resulting polypeptide is undesirable.