RSF1010 DERIVATIVE MOB-DEFICIENT PLASMID CONTAINING NO ANTIBIOTIC RESISTANCE GENE, BACTERIUM COMPRISING THE PLASMID AND METHOD FOR PRODUCING USEFUL METABOLITES

RSF1010 DERIVATIVE MOB-DEFICIENT PLASMID CONTAINING NO ANTIBIOTIC RESISTANCE GENE, BACTERIUM COMPRISING THE PLASMID AND METHOD FOR PRODUCING USEFUL METABOLITES

A Mob- plasmid having a RSF1010 replicon, comprising a gene coding for Rep protein and said plasmid has been modified to inactivate gene related to mobilization ability. The present invention also describes a bacterium having an ability to produce useful metabolites, comprising the plasmid and said...

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Bibliographische Detailangaben
Hauptverfasser: GULEVICH, ANDREY YURIEVICH, MIRONOV, ALEKSANDR, SERGEEVICH, KATASHKINA, JOANNA YOSIFOVNA, SKOROKHODOVA, ALEKSANDRA YURIEVNA, ERRAIS, LOPES, LUBOV, ZIMENKOV, DANILA VADIMOVICH, MASHKO, SERGEI, VLADIMIROVICH, BIRYUKOVA, IRINA, VLADIMIROVNA
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A Mob- plasmid having a RSF1010 replicon, comprising a gene coding for Rep protein and said plasmid has been modified to inactivate gene related to mobilization ability. The present invention also describes a bacterium having an ability to produce useful metabolites, comprising the plasmid and said bacterium lack active thymidylate synthase coded by thyA gene and thymidine kinase coded by tdk gene, and a method for producing useful metabolites, such as native or recombinant proteins, enzymes, L-amino acids, nucleosides and nucleotides, vitamins, using the bacterium.