APPARATUS AND METHOD FOR FLUORESCENT DETECTION IN BIOLOGICAL SAMPLES

Apparatus and method for fluorescent detection in biological samples in which non-uniformities in the emitted light due to the optical system rather than the specimens is corrected for by using specific optical elements. The apparatus comprises a source (105), illuminating a dichroic beam splitter (...

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Bibliographische Detailangaben
Hauptverfasser:	OLDHAM, MARK, BOEGE, STEVEN, ILKOVA, LIANA
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	Apparatus and method for fluorescent detection in biological samples in which non-uniformities in the emitted light due to the optical system rather than the specimens is corrected for by using specific optical elements. The apparatus comprises a source (105), illuminating a dichroic beam splitter (110) which reflects excitation light via a first optical element (130) onto a sample region (120) comprising a tray of samples (122). The emitted light is redirected through the first optical element (130) and beam splitter (110) to a detector (125). The first optical element (130) collimates and reduces non-uniformities in the emission light.