POLYMERIC NUCLEIC ACID HYBRIDIZATION PROBES

A novel polymeric nucleic acid probe improves detection sensitivity and specificity in a variety of hybridization platforms. The probe is made up of multiple short nucleic acid sequences (referred to as monomers) attached together to form a long polymeric probe for use in hybridization applications....

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Bibliographische Detailangaben
Hauptverfasser:	WHITAKER, TOM, J., PH., D, HURT, RICHARD, A
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A novel polymeric nucleic acid probe improves detection sensitivity and specificity in a variety of hybridization platforms. The probe is made up of multiple short nucleic acid sequences (referred to as monomers) attached together to form a long polymeric probe for use in hybridization applications. For applications requiring immobilization of the probes to a surface, the polymeric probes are similar to long DNA probes in that they can be immobilized to a variety of surfaces without need for a chemical modification to the end of the probe. Because target nucleic acids hybridize to the relatively short monomers in the polymeric probe, the polymeric probes are more specific than long DNA probes. In addition, polymeric probes also improve the signal-to-background ratio by increasing the number of accessible monomer oligonucleotide probes immobilized per unit area on a surface.