IN VITRO GENE EXPRESSION ASSAY FOR DETERMINING FLUOROPYRIMIDINE SENSITIVITY IN CHEMOTHERAPY

IN VITRO GENE EXPRESSION ASSAY FOR DETERMINING FLUOROPYRIMIDINE SENSITIVITY IN CHEMOTHERAPY

Methods of determining susceptibility to resistance to anti-cancer drugs such as fluoropyrimidines, antimetabolites and platinum-containing compounds are described. DNA microarray technology was used to investigate changes in the transcriptional profile of the MCF-7 breast cancer cell line following...

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Bibliographische Detailangaben
Hauptverfasser: LONGLEY, DAVID BRODERICK, JOHNSTON, PATRICK GERARD, MAXWELL, PAMELA JOAN
Format: Patent
Sprache:eng ; fre ; ger
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
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Zusammenfassung:Methods of determining susceptibility to resistance to anti-cancer drugs such as fluoropyrimidines, antimetabolites and platinum-containing compounds are described. DNA microarray technology was used to investigate changes in the transcriptional profile of the MCF-7 breast cancer cell line following treatment with fluoropyrimidines. Upregulated genes included the genes encoding Raf, K-ras, SLAP, phosphoinositide 3-kinase, COP9 homolog (HCOP9), apoptosis specific protein, APO-1 cell surface antigen, FLIP protein, cyclin G, CDC2 , cyclin­-dependent protein kinase -2, thymosin beta-10, myosin light chain (MLC-2), gelsolin, thymosin beta-4, SSAT, spermidine synthase, spermidine aminopropyltransferase, MAT-8 protein, annexin II, annexin IV, FGF receptor 2, transmembrane 4 superfamily protein, chaperonin 10, enoyl-CoA hydratase, nicotinamide nucleotide transhydrogenase, ribosomal protein S28, ribosomal protein L37, L23 mRNA for putative ribosomal protein, and ribosomal protein L7. Also described are assays for identifying novel chemotherapeutic agents.