PROCESS FOR PREPARING DIDEOXYINOSINE USING ADENOSINE DEAMINASE ENZYME

PROCESS FOR PREPARING DIDEOXYINOSINE USING ADENOSINE DEAMINASE ENZYME

A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time an...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: CHEN, JASON, G, STEIN, GREGORY, L, POLITINO, MICHAEL, ANDERSON, WENDY, L, LIU, SUO, W, BOYLE, ALFRED, W, FRANCESCHINI, THOMAS, SKONEZNY, PAUL, M
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SPIRITS
SUGARS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time and under conditions to produce a ddI solution; and (d) isolating the ddI from the ddI solution. Optionally, the ddI mother liquor is reused in subsequent runs to improve yield.