PROCESS FOR PRODUCING GLUCOSE DEHYDROGENASES

This invention relates to a process for preparing glucose dehydrogenase. The preparation process comprises introducing the DNA containing the sequence described in SEQ ID NO. : 1 coding for the alpha subunit, which has a glucose dehydrogenase activity, and the beta subunit, which is an electron-tran...

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Bibliographische Detailangaben
Hauptverfasser:	SODE, KOJI, KAWASE, SHIDO, HOSHIJIMA, MITSUHIRO, KUROSAKA, KEISUKE, YAMAOKA, HIDEAKI
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a process for preparing glucose dehydrogenase. The preparation process comprises introducing the DNA containing the sequence described in SEQ ID NO. : 1 coding for the alpha subunit, which has a glucose dehydrogenase activity, and the beta subunit, which is an electron-transfer protein, into a microorganism belonging to the genus Pseudomonas to create a transformant, and culturing this transformant to produce a first glucose dehydrogenase containing the beta subunit and a second glucose dehydrogenase not containing the beta subunit. The alpha subunit has a molecular weight of approximately 60kDa for example as determined by SDS-polyacrylamide gel electrophoresis under reducing conditions. The beta subunit has a molecular weight of approximately 43kDa for example as determined by SDS-polyacrylamide gel electrophoresis under reducing conditions.