A quantification method for integrated viruses

The invention relates to a method of detecting and/or quantifying a virus, such as HIV, integrated in a cellular genome, and to kits useful to perform this method. This two-step method calls for an anchored viral primer and Alu primers for a first round of PCR (Alu PCR) and a primer for the anchor a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	DELELIS, OLIVIER, LESTE-LASSERRE, THIERRY, PETIT, CAROLINE, PIERRE-BROCHE, SOPHIE, BRUSSEL, AUDREY, SONIGO, PIERRE
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method of detecting and/or quantifying a virus, such as HIV, integrated in a cellular genome, and to kits useful to perform this method. This two-step method calls for an anchored viral primer and Alu primers for a first round of PCR (Alu PCR) and a primer for the anchor along with another viral primer for the second round of PCR ("nested PCR").