METHOD FOR THE PURIFICATION OF A RHIZOMUCOR ASPARTIC PROTEASE

The invention disclose a method for purifying microbial protease, comprising:(i) providing an aqueous liquid sample containing a microbial protease, and a separation medium comprising a base matrix and a plurality of attached ligands that are capable of binding to microbial protease;(ii) contacting...

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Bibliographische Detailangaben
Hauptverfasser:	BECKHOVEN, VAN, RUDOLF, FRANCISCUS, WILHELMUS, C, NAEYE, THIERRY, JEAN-BERNARD
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROTEIN COMPOSITIONS FOR FOODSTUFFS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:	The invention disclose a method for purifying microbial protease, comprising:(i) providing an aqueous liquid sample containing a microbial protease, and a separation medium comprising a base matrix and a plurality of attached ligands that are capable of binding to microbial protease;(ii) contacting separation medium with the sample under conditions permitting binding of microbial protease to the separation medium; and (iii) desorbing microbial protease from the separation medium, wherein the base matrix is hydrophilic and the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp3-hybridised.