METHOD FOR THE DETECTION OF CYTOCHROME B MUTATIONS IN FUNGI

This invention relates to disgnostic methods for the detection of one or more cytochrome b mutations in fungi at the position corresponding to Saccharomyces cerevisiae cytochrome b residue 129 that leads to resistance to strobilurin analogues or compounds in the same cross resistance group using any...

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Bibliographische Detailangaben
Hauptverfasser:	WINDASS, JOHN, DAVID, BURBIDGE, JUDITH, MARY, STANGER, CAROLE, PATRICIA, CLEERE, SALLY, MICHELE
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to disgnostic methods for the detection of one or more cytochrome b mutations in fungi at the position corresponding to Saccharomyces cerevisiae cytochrome b residue 129 that leads to resistance to strobilurin analogues or compounds in the same cross resistance group using any (or a) single nucleotide polymorphism detection technique, preferably using either an allele specific amplilication technique such as the amplification refractory mutation system (ARMS) or preferably using an allele selective hybridisation probe technique such as Molecular Beacons or TaqMan. The invention also relates to mutation specific oligonucleotides for use in the method and to diagnostic kits containing these (oligonucleotides.