ASSAY FOR PREDICTING CELL ACTIVITY

The invention relates to a method of determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of: a) providing a sample of a cell population; b) dividing said sample into a first and a second part containing an equal number of cells; c) culturing t...

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Bibliographische Detailangaben
Hauptverfasser:	DA SILVA MADUREIRA MENDES, SANDRA, CLAUDIA, VAN BLITTERSWIJK, CLEMENS, ANTONI, DE BRUIJN, JOOST, DICK
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method of determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of: a) providing a sample of a cell population; b) dividing said sample into a first and a second part containing an equal number of cells; c) culturing the first part in the presence of an osteogenic stimulation factor; d) culturing the second part in the absence of an osteogenic stimulation factor; e) determining degrees of expression of a bone-specific protein; and f) comparing the degrees of expression of the bone-specific protein of the first part and the second part thereby providing a measure for the capacity of the bone cell population to induce bone formation in vivo.