Process for manufacturing a bone implant

Production of a bone implant comprises treating bone with formaldehyde, hydrochloric acid, formaldehyde, sodium thiosulfate, ammonia, ethanol, trishydroxymethylaminomethane and sodium chloride. Production of a bone implant comprises: (a) removing muscle, periosteum and marrow from long bones from donors aged 25-50; (b) covering the bones in an inert vessel with 5-10 volumes of a 0.2-0.5% formaldehyde solution prepared by dissolving 35 or 25% methanol-free extra-pure formaldehyde in a 0.85-0.95% sodium chloride solution; (c) keeping the bones in the vessel at 5-7degreesC for 7 days and changing the solution once a day; (d) mechanically working the bones to a size and shape adapted to a bone defect to be repaired, and removing any residual bone marrow; (e) keeping the bones in a 0.35% formaldehyde solution at 5-7degreesC for 24 hours; (f) keeping the bones in a 1-3% chemically pure hydrochloric acid solution until a predetermined degree of demineralization has been achieved; (g) keeping the bones in a 0.35% formaldehyde solution at 5-7degreesC for 24 hours; (h) treating the bones in a 1% sodium thiosulfate solution for 1 hour, shaking every 15 minutes; (i) keeping the bones in a 1.5% aqueous ammonia solution for 1 hour, shaking every 15 minutes; (j) keeping the bones in a 0.01-0.1% solution of chlorhexidine bigluconate solution in 70% ethanol for 48 hours at 5-7degreesC and changing the solution after 1, 6 and 24 hours; and (k) placing the bones in a 0.9% sodium chloride solution, changing the solution after 1, 2, 3, 4, 5, 6, 24 and 25 hours, and pouring off the solution. Zur Herstellung eines Knochenimplantats werden Knochen von Spendern in einer Reihe von Schritten mit Formaldehyd, Salzsäure, Natriumthiosulfat, Natriumchlorid, und weiteren Chemilkalien behandelt, um drin die angeborenen morphogenetischen Proteine zu bewahren.