Multi-step process for making a bone regeneration paste

Production of a composition for promoting bone regeneration comprises treating comminuted frozen bone alternately with formaldehyde and hydrochloric acid and then sequentially with sodium thiosulfate, ammonia, ethanol, trishydroxymethylaminomethane and sodium chloride. Production of a composition for promoting bone regeneration comprises: (a) removing muscle, periosteum and marrow from long bones from donors aged 25-50, dividing the bones into 2-4 cm pieces and drying the pieces; (b) freezing the pieces at -10 to -40degreesC for 18-24 hours; (c) dividing the pieces into small fragments (0.1-1 x 0.1-1 x 0.1-1 mm) at a temperature below 15degreesC; (d) covering the fragments in an inert vessel with 5-10 volumes of a 0.2-0.5% formaldehyde solution prepared by dissolving 35 or 25% methanol-free extra-pure formaldehyde in a 0.85-0.95% sodium chloride solution; (e) keeping the fragments in the formaldehyde solution at 2-15degreesC for 48 hours, changing the solution after 1, 2, 4, 6 and 24 hours and shaking the vessel for about 1 minute every hour between changes; (f) pouring off the formaldehyde solution and pouring in a 1-3% chemically pure hydrochloric acid (HCl) solution; (g) pouring off the HCl solution after 22-26 hours and pouring in a 0.2-0.5% formaldehyde solution; (h) pouring off the formaldehyde solution after 22-26 hours and pouring in a 1-3% HCl solution; (i) repeating steps (g) and (h) 12-14 times while keeping the temperature in the vessel below 15degreesC; (j) pouring in a 1% sodium thiosulfate solution, allowing it to act for 5 minutes and pouring it off; (k) pouring in a 1.5% aqueous ammonia solution, allowing it to act for 5 minutes and pouring it off; (l) pouring in a 0.01-0.1% solution of chlorhexidine bigluconate solution in 70% ethanol, allowing it to act for 10-120 minutes and pouring it off; (m) pouring in 70% ethanol and changing it after 4-8 hours and again after 24-48 hours; (n) pouring off the ethanol and pouring in a 3.6-3.7% trishydroxymethylaminomethane (Tris) solution; (o) pouring off the Tris solution after 1 hour and replacing it with a 0.9% sodium chloride solution; (p) changing the sodium chloride solution after 1, 2, 3, 4, 5 and 6 hours (+/- 10 minutes) and after 24 hours (+/- 1 hour); and (q) pouring off the sodium chloride solution about 1 hour after the last change and draining the product. An Independent claim is also included for bone paste produced by the above process.