CULTURED EPIDERMAL CELL SHEET, LAMINATED CULTURED SKIN SHEET AND PROCESS FOR PRODUCING THE SAME

Cells are cultured on a cell culture support having a substrate surface coated with a temperature-responsive polymer whose upper or lower critical solution temperature in water is 0 &tilde& 80 DEG C, optionally forming multiple cultured cell layers by a conventional technique, and subsequent...

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Bibliographische Detailangaben
Hauptverfasser:	KUSHIDA, AI, OKANO, TERUO, KONNO, CHIE, KIKUCHI, AKIHIKO, UTSUMI, MIKA, YAMATO, MASAYUKI
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Cells are cultured on a cell culture support having a substrate surface coated with a temperature-responsive polymer whose upper or lower critical solution temperature in water is 0 &tilde& 80 DEG C, optionally forming multiple cultured cell layers by a conventional technique, and subsequently: (1) the temperature of the culture solution is brought to above the upper critical solution temperature or below the lower critical solution temperature; (2) the cultured epidermal cell sheet or multi-layered skin sheet is brought into close contact with the polymer membrane; and (3) the sheet is peeled off together with the polymer membrane. The thus obtained epidermal cultured cell sheet and multi-layered cultured skin sheet do not degrade E-cadherin or laminin 5 as contrasted with Dispase treatment and they still involve very few structural defects; these sheets hence hold great promise in clinical applications such as skin grafting.