BACILLUS TRANSFORMATION, TRANSFORMANTS AND MUTANT LIBRARIES

The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.

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Bibliographische Detailangaben
Hauptverfasser: SCHELLENBERGER, VOLKER, SELIFONOVA, OLGA, V, LEE, EDWIN, W, DIAZ-TORRES, MARIA, R, MORRISON, THOMAS, B
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.