NITROREDUCTASE REPORTER GENE ASSAY INVOLVING FLUORESCENCE ENHANCEMENT

Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO 2 group characterised by the reduction of the at least one NO 2 group to NHOH or NH 2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO 2 group can be used...

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Bibliographische Detailangaben
Hauptverfasser:	DAVIES, BETH, MICHAEL, NIGEL PAUL, BRIGGS, MARK, THOMAS, NICHOLAS, MILLAR, VALERIE
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DYES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LAKES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MORDANTS MUTATION OR GENETIC ENGINEERING NATURAL RESINS ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES PAINTS PHYSICS POLISHES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO 2 group characterised by the reduction of the at least one NO 2 group to NHOH or NH 2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO 2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.