COLOUR LABELLED OLIGONUCLEOTIDE FOR LABELLING A NUCLEIC ACID MOLECULE

The invention relates to an oligonucleotide which serves as a nucleic acid probe comprising a loop section with a loop sequence complementary to the target sequence of the nucleic acid molecule; a stem section arranged on both ends of the loop section for hybridizing with each other for closing the...

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Bibliographische Detailangaben
Hauptverfasser:	WOLFRUM, JUERGEN, SAUER, MARKUS
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an oligonucleotide which serves as a nucleic acid probe comprising a loop section with a loop sequence complementary to the target sequence of the nucleic acid molecule; a stem section arranged on both ends of the loop section for hybridizing with each other for closing the oligonucleotides thereby forming a loop, and wherein one of the stem sections is labeled with fluorescent color, such that when the loop section hybridizes with the target sequence of the nucleic acid, the oligonucleotide opens and the distance between the fluorophore and a quencher nuleoside increases which thereby prevents quenching of the fluorescence and the strength of fluorescence when measured indicates the presence of the nucleic acid with the target sequence.