Mutant B-typ DNA polymerases exhibiting improved performance in PCR

Mutant B-typ DNA polymerases exhibiting improved performance in PCR

Claimed are thermostable mutants of B-type DNA polymerases having a Y-GG/A amino acid motif between the N-terminal 3'-5'-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suita...

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Hauptverfasser: ROSSI, MOSE DR, BOEHLKE, KRISTINA, ANTRANIKIAN, GARABED DR, FREY, BRUNO DR, SOBEK, HARALD DR, PISANI, FRANCESCA MARIA DR
Format: Patent
Sprache:eng ; fre ; ger
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BIOCHEMISTRY
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Zusammenfassung:Claimed are thermostable mutants of B-type DNA polymerases having a Y-GG/A amino acid motif between the N-terminal 3'-5'-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR.