Method and device for detection of nucleic acid using total internal reflectance

An apparatus for detecting a target molecule in a specific binding assay using a fluorescence total internal reflection (TIR) element in a sealed static-volumetric reaction vessel is provided wherein the reaction vessel wall and TIR element surface are separated from one another by a distance which...

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Bibliographische Detailangaben
Hauptverfasser:	KHALIL, OMAR S, BOUMA, STANLEY R, PABICH, EDWARD K
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	An apparatus for detecting a target molecule in a specific binding assay using a fluorescence total internal reflection (TIR) element in a sealed static-volumetric reaction vessel is provided wherein the reaction vessel wall and TIR element surface are separated from one another by a distance which precludes capillary migration of an aqueous fluid.